Human GPIIb/IIIa (Glycoprotein IIb/IIIa) ELISA Kit - ELK1688

Human GPIIb/IIIa (Glycoprotein IIb/IIIa) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1688-48, ELK1688-96, ELK1688-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: GP-IIb/IIIa

Assay Type: Sandwich

Sensitivity: 0.058 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: CD and Adhesion molecule; Metabolic pathway; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GPIIb/IIIa. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GPIIb/IIIa. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GPIIb/IIIa, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GPIIb/IIIa in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only