Product Description

Human GREM2 (Gremlin 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0124-48, ELK0124-96, ELK0124-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: Cysteine knot superfamily 1, BMP antagonist 2, Protein related to DAN and cerberus, PRDC, Cktsf1b2

Sensitivity: 11.3 pg/mL

Assay Type: Sandwich

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Signal Transduction, Tumor Immunity, Developmental Science, Bone Metabolism

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GREM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GREM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GREM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GREM2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9H772

Research Use Only