Product Description

Human GZMA (Granzyme A) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4608MS-48, ELK4608MS-96, ELK4608MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GZMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GZMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GZMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GZMA in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternate Names: CTLA3, HFSP, HF, H factor, Granzyme 1, Fragmentin-1, Hanukkah factor, Cytotoxic T-Lymphocyte-Associated Serine Esterase 3, CTL tryptase, Cytotoxic T-lymphocyte proteinase 1

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase, Infection Immunity, Immune Molecule

Assay Length: 3.5h

Sensitivity: 0.113 ng/mL

Standard: 20 ng/mL

Detection Method: 0.32-20 ng/mL

UniProt: P12544

Research Use Only