Product Description

Human HBb (Hemoglobin Beta) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK4071-48, ELK4071-96, ELK4071-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBb in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: HB-B, HBD, CD113t-C, Beta Globin, Spinorphin

Reactivity: Human

Sample Type: Serum, Plasma and erythrocyte lysates

Assay Type: Competitive Inhibition

Research Areas: Hematology

Assay Length: 2h

Sensitivity: 2.82 pg/mL

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

UniProt: P68871

Research Use Only