Human HBF (Fetal Hemoglobin) ELISA Kit - ELK10343

Human HBF (Fetal Hemoglobin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10343-48, ELK10343-96, ELK10343-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: hemoglobin F; Foetal Haemoglobin

Assay Type: Competitive Inhibition

Sensitivity: 0.99 µg/mL

Standard: 200 µg/mL

Detection Range: 3.13-200 µg/mL

Sample Type: serum, plasma, erythrocyte lysates and other biological fluids

Assay Length: 2h

Research Areas: Hematology;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBF in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only