Human HBg1 (Hemoglobin Gamma 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4070-48, ELK4070-96, ELK4070-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: HB-G1; HBGA; HBGR; HSGGL1; Gamma-1-globin
Assay Type: Competitive Inhibition
Sensitivity: 202.8 ng/mL
Standard: 30000 ng/mL
Detection Range: 468.75-30000 ng/mL
Sample Type: Serum, plasma, erythrocyte lysates
Assay Length: 2h
Research Areas: Hematology;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HBg1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBg1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBg1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P69891
Research Use Only