Product Description

Human HBP (Heparin-Binding Protein) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11782-48, ELK11782-96, ELK11782-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: AZU, AZAMP, AZU1, HBP, HUMAZUR, NAZC, CAP37, Cationic Antimicrobial Protein 37, Heparin-Binding Protein, Neutrophil Azurocidin

Sensitivity: 3.53 pg/mL

Assay Type: Sandwich

Standard: 1500 pg/mL

Detection Range: 23.44-1500 pg/mL

Sample Type: Serum

Assay Length: 3.5h

Research Area: Enzyme And Kinase, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HBP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HBP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HBP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HBP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only