Product Description

Human HECTD3 (E3 Ubiquitin-Protein Ligase HECTD3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0325-48, ELK0325-96, ELK0325-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: HECT domain-containing protein 3, HECT-type E3 ubiquitin transferase HECTD3

Sensitivity: 0.065 ng/mL

Assay Type: Sandwich

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Length: 3.5h

Research Area: Enzyme And Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HECTD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HECTD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HECTD3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HECTD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q5T447

Research Use Only