Product Description

Human HMW Adiponectin (High Molecular Weight Adiponectin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11456-48, ELK11456-96, ELK11456-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: HMW Adiponectin,

Sensitivity: 1.23 ng/mL

Assay Type: Sandwich

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Metabolic Disease

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HMW Adiponectin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HMW Adiponectin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HMW Adiponectin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HMW Adiponectin in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only