Product Description

Human Holo TC (Holotranscobalamin) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK0720-48, ELK0720-96, ELK0720-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human Holo TC. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human Holo TC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human Holo TC in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Holo-TC

Reactivity: Human

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Metabolic Pathway, Nutrition Metabolism

Assay Length: 2.5h

Sensitivity: 0.045 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Research Use Only