Product Description

Human hs-Hepc25 (high sensitivity Hepcidin 25) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK9112MS-48, ELK9112MS-96, ELK9112MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-Hepc25. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-Hepc25. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-Hepc25, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-Hepc25 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Hamp, HEPC, HEPC_HUMAN, Hepc20, Hepc25, HEPCIDIN, Hepcidin 20, Hepcidin 25, Hepcidin antimicrobial peptide, Hepcidin-20, Hepcidin25, HFE2, HFE2B, LEAP 1, LEAP-1, LEAP1, Liver expressed antimicrobial peptide, Liver-expressed antimicrobial peptide 1, PLTR, Putative liver tumor regressor,

Reactivity: Human

Sample Type: Serum, Plasma, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Cardiovascular, Signal Transduction, Cardiovascular, Metabolism

Assay Length: 3.5h

Sensitivity: 0.61 ng/mL

Standard: 100 ng/mL

Detection Range: 1.57-100 ng/mL

Research Use Only