Human HVD3 (25-Hydroxyvitamin D3) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK8625-48, ELK8625-96, ELK8625-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: 25-HVD3; 25HVD3; Calcifediol; Calcidiol; H-VD3; 25-Hydroxycholecalciferol; 25-Hydroxy Vitamin D3; 25 (OH)D
Assay Type: Competitive Inhibition
Sensitivity: 0.88 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Reproductive science; Genetic science; Nutrition metabolism; Bone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human HVD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HVD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HVD3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only