Human IA-2 Ab (Insulinoma associated antigen 2 Autoantibody) ELISA Kit - ELK0858

Human IA-2 Ab (Insulinoma associated antigen 2 Autoantibody) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0858-48, ELK0858-96, ELK0858-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: IA2 Ab

Assay Type: Sandwich

Sensitivity: 26.8 U/mL

Standard: 4000 U/mL

Detection Range: 62.5-4000 U/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IA-2 Ab. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IA-2 Ab. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IA-2 Ab, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IA-2 Ab in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only