Product Description

Human IAPP (Islet Amyloid Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4831-48, ELK4831-96, ELK4831-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: IAP, DAP, Amylin, Insulinoma Amyloid Polypeptide, Diabetes-associated peptide

Sensitivity: 2.47 pg/mL

Assay Type: Competitive Inhibition

Standard: 500 pg/mL

Detection Range: 7.82-500 pg/mL

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernates, Other Biological Fluids

Assay Length: 2h

Research Area: Metabolic Pathway, Endocrinology, Hepatology

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IAPP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IAPP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IAPP in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P10997

Research Use Only