Human IGFIIE (Pro-Insulin Like Growth Factor II E-Peptide) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK4859-48, ELK4859-96, ELK4859-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Preptin; Proinsulin-Like Growth Factor-II
Assay Type: Competitive Inhibition
Sensitivity: 13.3 pg/mL
Standard: 3000 pg/mL
Detection Range: 46.88-3000 pg/mL
Sample Type: Serum, plasma, other biological fluids
Assay Length: 2h
Research Areas: Infection immunity; Hepatology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human IGFIIE. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IGFIIE. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IGFIIE in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only