Product Description

Human IL-6R (Interleukin 6 Receptor) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11855-48, ELK11855-96, ELK11855-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: IL-6R,CD126, gp80, IL6R, IL-6R-1, IL-6R-Alpha, IL6RA, IL-6 receptor subunit alpha, Membrane glycoprotein 80,

Sensitivity: 0.45 ng/mL

Assay Type: Sandwich

Standard: 30 ng/mL

Detection Range: 0.47-30 ng/mL

Sample Type: Serum, Plasma

Assay Length: 3.5h

Research Area: Signal Transduction, Cd And Adhesion Molecule, Infection Immunity

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human IL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human IL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human IL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human IL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P08887

Research Use Only