Human IL24 (Interleukin 24) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2454MS-48, ELK2454MS-96, ELK2454MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: V75; VN; VNT; Serum Spreading Factor; Somatomedin B; Complement S-Protein
Assay Type: Sandwich
Sensitivity: 1.03 µg/mL
Standard: 200 µg/mL
Detection Range: 3.13-200 µg/mL
Sample Type: Plasma.
Assay Length: 3.5h
Research Areas: Signal transduction; Metabolic pathway; Tumor immunity; Hepatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse VTN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse VTN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse VTN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse VTN in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P29788
Research Use Only