Human IL5 (Interleukin 5) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2277MS-48, ELK2277MS-96, ELK2277MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa
Assay Type: Sandwich
Sensitivity: 12.7 pg/mL
Standard: 1000 ng/mL
Detection Range: 15.63-1000 ng/mL
Sample Type: Plasma
Assay Length: 3.5h
Research Areas: Metabolic pathway; Infection immunity; Hematology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat F7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat F7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat F7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat F7 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8K3U6
Research Use Only