Product Description

Human KLK12 (Kallikrein 12) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3389-48, ELK3389-96, ELK3389-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: KLK-L5; KLKL5; Kallikrein-Related Peptidase 12; Kallikrein-like protein 5

Assay Type: Competitive Inhibition

Sensitivity: 6.83 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Sample Type: Serum, plasma, other biological fluids

Assay Length: 2h

Research Areas: Enzyme and Kinase

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK12. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK12. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK12 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9UKR0

Research Use Only