Human KLK8 (Kallikrein 8) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1851-48, ELK1851-96, ELK1851-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: NP; HNP; NRPN; PRSS19; TADG14; BSP1; Neuropsin; Ovasin; Brain Serine Protease 1; Tumor Associated Differentially Expressed Gene 14; Serine protease 19
Assay Type: Competitive Inhibition
Sensitivity: 216.4 pg/mL
Standard: 50000 pg/mL
Detection Range: 781.25-50000 pg/mL
Sample Type: Serum, plasma and other biological fluids
Assay Length: 2h
Research Areas: Enzyme and Kinase; Tumor immunity; Neuroscience;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human KLK8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLK8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLK8 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: O60259
Research Use Only