Product Description

Human LAB7-2 (B-Lymphocyte Activation Antigen B7-2) Microsample ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK1606MS-48, ELK1606MS-96, ELK1606MS-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LAB7-2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LAB7-2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LAB7-2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LAB7-2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: CD86, B7-2, B70, FUN-1, CD28LG2, CD28 Antigen Ligand 2, B7-2 Antigen, CTLA-4 counter-receptor B7.2

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction, Cd and Adhesion Molecule, Tumor Immunity, Infection Immunity, Immune Molecule

Assay Length: 3.5h

Sensitivity: 0.056 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Research Use Only