Product Description

Human LFA3 (Lymphocyte Function Associated Antigen 3) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK10469-48, ELK10469-96, ELK10469-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: CD58, Ag3, Surface glycoprotein LFA-3

Sensitivity: 12.8 pg/mL

Assay Type: Sandwich

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, Plasma, Cell Lysatesand Other Biological Fluids

Assay Length: 3.5h

Research Area: Cd And Adhesion Molecule, Tumor Immunity, Infection Immunity, Immune Molecule

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LFA3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LFA3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LFA3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LFA3 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P19256

Research Use Only