Human LTb (Lymphotoxin Beta) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK2068MS-48, ELK2068MS-96, ELK2068MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: GSN; AGEL; ADF; Brevin; Gelsolin Amyloidosis, Finnish Type; Actin-depolymerizing factor
Assay Type: Sandwich
Sensitivity: 2.2 µg/mL
Standard: 500 µg/mL
Detection Range: 7.82-500 µg/mL
Sample Type: Serum, plasma and other biological fluids.
Assay Length: 3.5h
Research Areas: Tumor immunity; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GS. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GS in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P13020
Research Use Only