Human LUM (Lumican) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK2021-48, ELK2021-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: LDC; SLRR2D; KSPG lumican; Keratan sulfate proteoglycan lumican
Species: Human
Assay Type: Sandwich
Sensitivity: 0.124 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 3.5h
Research Areas: Metabolic pathway; Tumor immunity; Infection immunity; Bone metabolism; Dermatology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LUM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LUM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LUM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LUM in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P51884
Research Use Only.