Human MIg (Monokine Induced By Interferon Gamma) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1284MS-48, ELK1284MS-96, ELK1284MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: LCN2; p25; Lipocalin 2; Oncogene 24p3; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Siderocalin LCN2
Assay Type: Sandwich
Sensitivity: 5.6 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma
Assay Length: 1.5h
Research Areas: Infection immunity; Urology;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NGAL, and the Human NGAL standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Human NGAL. After TMB substrate solution is added, only those wells that contain Human NGAL and HRP-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NGAL in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P80188
Research Use Only