Human MIP1a (Macrophage Inflammatory Protein 1 Alpha) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK1115MS-48, ELK1115MS-96, ELK1115MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: sHLA-G
Assay Type: Sandwich
Sensitivity: 0.066 ng/mL
Standard: 10 ng/mL
Detection Range: 0.16-10 ng/mL
Sample Type: serum, plasma, tissue homogenates and other biological fluids
Assay Length: 3.5h
Research Areas: Apoptosis; Tumor immunity; Infection immunity; Immune molecule; Hematology; Reproductive science; Immunodeficiency; Autoimmunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sHLA-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sHLA-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sHLA-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sHLA-G in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only