Human MOTS-c (Mitochondrial Open Reading Frame Of The 12S rRNA-c) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK7627-48, ELK7627-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: MOTS-c
Species: Human
Assay Type: Competitive Inhibition
Sensitivity: 0.99 ng/mL
Standard: 200 ng/mL
Detection Range: 3.13-200 ng/mL
Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates and other Biological Fluids
Assay length: 2h
Research Areas: Developmental science
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MOTS-c protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MOTS-c. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MOTS-c in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: A0A0C5B5G6
Research Use Only.