Human MR-ProADM (Mid-regional pro-adrenomedullin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9597-48, ELK9597-96, ELK9597-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: MR-ProADM
Assay Type: Sandwich
Sensitivity: 9.17 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Endocrinology; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-ProADM. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-ProADM. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-ProADM, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-ProADM in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only