Human MR-proANP (midregional pro-atrial natriuretic peptide) Microsample ELISA Kit - ELK0872MS

Human MR-proANP (midregional pro-atrial natriuretic peptide) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0872MS-48, ELK0872MS-96, ELK0872MS-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: MR-proANP

Assay Type: Sandwich

Sensitivity: 0.05 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Endocrinology; Cardiovascular biology;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MR-proANP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MR-proANP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MR-proANP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MR-proANP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only