Product Description

Human MTX (Methotrexate) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK8649-48, ELK8649-96, ELK8649-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human MTX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MTX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MTX in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: Methotrexate, Amethopterin

Reactivity: Human

Sample Type: Serum, Plasma, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Competitive Inhibition

Research Areas: Cancer

Assay Length: 2h

Sensitivity: 4.57 ng/mL

Standard: 1000 ng/mL

Detection Range: 15.63-1000 ng/mL

Research Use Only