Human MYO (Myoglobin) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10220MS-48, ELK10220MS-96, ELK10220MS-5x96
Citations, Manuals and MSDS Available upon request.
Reactivity: Human
Alternative Names: IL-6R; CD126; gp80; IL6R; IL-6R-1; IL-6R-Alpha; IL6RA; IL-6 receptor subunit alpha; Membrane glycoprotein 80; IL6R; Interleukin 6 Receptor
Assay Type: Sandwich
Sensitivity: 0.78 ng/mL
Standard: 120 ng/mL
Detection Range: 1.88-120 ng/mL
Sample Type: Serum, plasma, tissue homogenates, urine and other biological fluids
Assay Length: 3.5h
Research Areas: Signal transduction; CD and Adhesion molecule; Infection immunity;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human sIL-6R. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human sIL-6R. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human sIL-6R, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human sIL-6R in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only