Product Description

Human MYOF (Myoferlin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK11843-48, ELK11843-96, ELK11843-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: Myoferlin,MYOF,Fer-1-like protein 3,FER1L3,,KIAA1207

Sensitivity: 0.19 ng/mL

Assay Type: Sandwich

Standard: 40 ng/mL

Detection Range: 0.63-40 ng/mL

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids.

Assay Length: 3.5h

Research Area: Tumor Immunity, Cardiovascular Biology

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MYOF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MYOF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MYOF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MYOF in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q9NZM1

Research Use Only