Human NEB (Noradrenaline Bitartas) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0075-48, ELK0075-96, ELK0075-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: (R)- (−)-norepinephrine; (R)-4- (2-amino-1-hydroxyethyl)-1,2-benzenediol; (R)-noradrenaline
Assay Type: Competitive Inhibition
Sensitivity: 2.1 ng/mL
Standard: 400 ng/mL
Detection Range: 6.25-400 ng/mL
Sample Type: Serum, plasma, other biological fluids
Assay Length: 3.5h
Research Areas: Signal Transduction
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human NEB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEB in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only