Product Description

Human NEDD4-L (E3 ubiquitin-protein ligase NEDD4-like) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK9502-48, ELK9502-96, ELK9502-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Human

Alternate Names: NED4L,NEDD4 2

Sensitivity: 0.124 ng/mL

Assay Type: Sandwich

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, Plasma, Tissue Homogenates, Other Biological Fluids

Assay Length: 3.5h

Research Area: Enzyme And Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NEDD4-L. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NEDD4-L. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NEDD4-L, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NEDD4-L in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: Q96PU5

Research Use Only