Human NiV-G (Nipah Virus Glycoprotein G) ELISA Kit
Size: 48T, 96T, 96T*5
Manual, SDS and citations available upon request.
Catalogue Numbers: ELK11852-48, ELK11852-96, ELK11852-96*5
Reactivity: Human
Alternative Names: Nipah Virus Glycoprotein G
Assay Type: Sandwich
Sensitivity: 8.4 pg/mL
Standard: 1000 pg/mL
Detection Range: 15.63-1000 pg/mL
Sample Type: Serum, plasma
Assay Length: 3.5h
Research Area: Microbiology
Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NiV-G. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NiV-G. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NiV-G, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NiV-G in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only