Human NKX6-1 (NK6 Homeobox Protein 1) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK3376-48, ELK3376-96, ELK3376-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: NKX6A; Nkx6.1; NK6 Transcription Factor Related,Locus 1; Homeobox protein NK-6 homolog A
Assay Type: Sandwich
Sensitivity: 0.129 ng/mL
Standard: 20 ng/mL
Detection Range: 0.32-20 ng/mL
Sample Type: Tissue homogenates, cell lysates and other biological fluids.
Assay Length: 3.5h
Research Areas: Signal transduction
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX6-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX6-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P78426
Research Use Only