Product Description

Human NKX6-1 (NK6 Homeobox Protein 1) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK3376-48, ELK3376-96, ELK3376-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NKX6-1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NKX6-1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NKX6-1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NKX6-1 in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: NKX6A, Nkx6.1, NK6 Transcription Factor Related, Locus 1, Homeobox protein NK-6 homolog A

Reactivity: Human

Sample Type: Tissue Homogenates, Cell Lysates, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Signal Transduction

Assay Length: 3.5h

Sensitivity: 0.129 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

UniProt: P78426

Research Use Only