Human NP-B (Neuropeptide B) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0059-48, ELK0059-96, ELK0059-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: L7; PPNPB; PPL7; NPB
Assay Type: Sandwich
Sensitivity: 26.3 pg/mL
Standard: 5000 pg/mL
Detection Range: 78.13-5000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Neuroscience; Hormone metabolism;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP-B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP-B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP-B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP-B in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: Q8NG41
Research Use Only