Human NP (Influenza B Nucleoprotein) ELISA Kit - ELK11851

Human NP (Influenza B Nucleoprotein) ELISA Kit

Size: 48T, 96T, 96T*5

Manual, SDS and citations available upon request.

Catalogue Numbers: ELK11851-48, ELK11851-96, ELK11851-96*5

Reactivity: Human

Alternative Names: Nucleoprotein; Nucleocapsid protein; Protein N; NP

Assay Type: Sandwich

Sensitivity: 28 pg/mL

Standard: 8 ng/mL

Detection Range: 0.13-8 ng/mL

Sample Type: Serum, plasma

Assay Length: 3.5h

Research Area: Microbiology

Test principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only