Human ORAI2 (ORAI Calcium Release-Activated Calcium Modulator 2) ELISA Kit - ELK0427

Human ORAI2 (ORAI Calcium Release-Activated Calcium Modulator 2) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0427-48, ELK0427-96, ELK0427-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: C7orf19; CBCIP2; TMEM142B; CAP-binding protein complex-interacting protein 2; Transmembrane protein 142B

Assay Type: Sandwich

Sensitivity: 0.12 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: serum, plasma, urine, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Length: 3.5h

Research Areas: Signal transduction

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ORAI2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ORAI2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ORAI2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ORAI2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: ORAI2

Research Use Only