Human OX (Orexin) ELISA Kit - ELK4722

Human OX (Orexin) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4722-48, ELK4722-96, ELK4722-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: HCRT; PPOX; Orexin A; Hypocretin 1; Orexin B; Hypocretin 2; Preproorexin; Hypocretin Neuropeptide Precursor

Assay Type: Competitive Inhibition

Sensitivity: 4.6 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: serum, plasma, other biological fluids

Assay Length: 2h

Research Areas: Neuroscience

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human OX. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human OX. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human OX in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O43612

Research Use Only