Product Description

Human p-AMPK (Phosphorylated Adenosine Monophosphate Activated Protein Kinase) ELISA Kit

Sizes: 48T, 96T 96T*5

Catalogue Number: ELK11308-48, ELK11308-96, ELK11308-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human p-AMPK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human p-AMPK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human p-AMPK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human p-AMPK in the samples is then determined by comparing the OD of the samples to the standard curve.

Alternative Names: p-PRKAA1, p-AMPK, p-AMPKa1, p-AMPK alpha 1

Reactivity: Human

Sample Type: Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernatant, Other Biological Fluids

Assay Type: Sandwich

Research Areas: Enzyme and Kinase

Assay Length: 3.5h

Sensitivity: 0.121 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Research Use Only