Human P-Cadherin (Cadherin, Placental) Microsample ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK9588MS-48, ELK9588MS-96, ELK9588MS-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: CDH3; CDHP; HJMD; PCAD; Cadherin 3; type 1
Assay Type: Sandwich
Sensitivity: 36 pg/mL
Standard: 4000 pg/mL
Detection Range: 62.50-4000 pg/mL
Sample Type: serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Length: 3.5h
Research Areas: Signal Transduction; Neuroscience
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human P-Cadherin. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human P-Cadherin. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human P-Cadherin, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human P-Cadherin in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P22223
Research Use Only