Human P2RY12 (Purinergic Receptor P2Y, G Protein Coupled 12) Microsample ELISA Kit - ELK4385MS

Human P2RY12 (Purinergic Receptor P2Y, G Protein Coupled 12) Microsample ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK4385MS-48, ELK4385MS-96, ELK4385MS-5x96

Citations, Manuals and MSDS Available upon request.

Reactivity: Human

Alternative Names: Don-1; HRG2; NTAK; Neural And Thymus Derived Activator For ErbB Kinases; Divergent Of Neuregulin-1; Pro-neuregulin-2, membrane-bound isoform

Assay Type: Sandwich

Sensitivity: 0.061 ng/mL

Standard: 10 ng/mL

Detection Range: 0.16-10 ng/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Neuro science;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human NRG2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human NRG2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human NRG2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human NRG2 in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O14511

Research Use Only