Human PDE4A (Phosphodiesterase 4A, cAMP Specific) ELISA Kit - ELK0902

Human PDE4A (Phosphodiesterase 4A, cAMP Specific) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK0902-48, ELK0902-96, ELK0902-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PDE4; DPDE2; PDE46

Assay Type: Sandwich

Sensitivity: 26.7 pg/mL

Standard: 4000 pg/mL

Detection Range: 62.5-4000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids.

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDE4A. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDE4A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDE4A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDE4A in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P27815

Research Use Only