Human PDGFB (Platelet Derived Growth Factor Subunit B) ELISA Kit - ELK1856

Human PDGFB (Platelet Derived Growth Factor Subunit B) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK1856-48, ELK1856-96, ELK1856-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: PDGF-B; PDGF2; SIS; SSV; C-sis; Simian Sarcoma Viral (v-sis)Oncogene Homolog; Platelet Derived Growth Factor Beta Polypeptide; Proto-oncogene c-Sis; Becaplermin

Assay Type: Sandwich

Sensitivity: 6.2 pg/mL

Standard: 1000 pg/mL

Detection Range: 15.63-1000 pg/mL

Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.

Assay Length: 3.5h

Research Areas: Cytokine;

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PDGFB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PDGFB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PDGFB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PDGFB in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: P01127

Research Use Only