Human PEA (Pseudomonas Exotoxin A) ELISA Kit
Sizes: 48T, 96T
Catalogue Numbers: ELK0679-48, ELK0679-96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Pseudomonas Exotoxin A
Species: Human
Assay Type: Sandwich
Sensitivity: 15.6 pg/mL
Standard: 2000 pg/mL
Detection Range: 31.25-2000 pg/mL
Sample Type: Serum, Plasma, Tissue Homogenates and other Biological Fluids
Assay length: 3.5h
Research Areas: Tumor immunity
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PEA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PEA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PEA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PEA in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only.