Human pERK (Phospho Extracellular Signal Regulated Kinase) ELISA Kit - ELK8541

Human pERK (Phospho Extracellular Signal Regulated Kinase) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK8541-48, ELK8541-96, ELK8541-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: MAPK1; ERK; ERK-2; ERK2; ERT1; MAPK2; P42MAPK; PRKM1; PRKM2; p38; p40; p41; p41mapk; p42-MAPK; mitogen-activated protein kinase 1

Assay Type: Sandwich

Sensitivity: 11.36 pg/mL

Standard: 2000 pg/mL

Detection Range: 31.25-2000 pg/mL

Sample Type: Serum, plasma, tissue homogenates and other biological fluids

Assay Length: 3.5h

Research Areas: Regulating translation

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human pERK. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human pERK. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human pERK, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human pERK in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only