Human PGF2α (Prostaglandin F2 Alpha) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK10032-48, ELK10032-96, ELK10032-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: PGF2A; PGF2-A; PG-F2a
Assay Type: Competitive Inhibition
Sensitivity: 3.65 pg/mL
Standard: 800 pg/mL
Detection Range: 12.5-800 pg/mL
Sample Type: Serum, plasma, saliva and other biological fluids
Assay Length: 2h
Research Areas: Metabolic pathway; Cardiovascular biology; Hormone metabolism;
Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PGF2α. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PGF2α. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PGF2α in the samples is then determined by comparing the OD of the samples to the standard curve.
Research Use Only