Human PIIICP (Procollagen III C-Terminal Propeptide) ELISA Kit - ELK5493

Human PIIICP (Procollagen III C-Terminal Propeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK5493-48, ELK5493-96, ELK5493-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: P3CP; C-Propeptide Of Type III Procollagen; Procollagen III Carboxy Terminal Propeptide

Assay Type: Competitive Inhibition

Sensitivity: 0.96 ng/mL

Standard: 200 ng/mL

Detection Range: 3.13-200 ng/mL

Sample Type: serum, plasma, cell lysates, cell culture supernates and other biological fluids

Assay Length: 2h

Research Areas: Metabolic pathway; Bone metabolism;

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Human PIIICP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIIICP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIIICP in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only