Human PIK3Cd (Phosphoinositide-3-Kinase Catalytic Delta Polypeptide) ELISA Kit - ELK3401

Human PIK3Cd (Phosphoinositide-3-Kinase Catalytic Delta Polypeptide) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK3401-48, ELK3401-96, ELK3401-5x96

Citations, Manuals and MSDS Available upon request.

Alternative Names: p110D; P110δ; Phosphoinositide-3-Kinase C; Phosphatidylinositol 4,5-bisphosphate 3-kinase 110 kDa catalytic subunit delta

Assay Type: Sandwich

Sensitivity: 0.118 ng/mL

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: tissue homogenates, cell lysates and other biological fluids

Assay Length: 3.5h

Research Areas: Enzyme and Kinase

Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PIK3Cd. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PIK3Cd. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PIK3Cd, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PIK3Cd in the samples is then determined by comparing the OD of the samples to the standard curve.

UniProt ID: O00329

Research Use Only